Sartorius adsorber removes chromatography contaminants

Sartobind Phenyl membrane adsorbers from Sartorius Stedim Biotech allow increased flow rates and faster processes and can be used for process upgrades in the purification and polishing of therapeutic proteins.

Sartobind Phenyl works similar to an HIC ligand adsorber, but behaves like a conventional HIC matrix. The hydrophobic phenyl ligand is covalently attached to the >3 µm cellulose membrane. Target molecules bind to the membrane with similar binding capacities and at the same buffer conditions as with conventional HIC columns. However, the flow rates can be increased at least 10-fold to allow a faster process upgrade in purification and polishing of therapeutic proteins. The resolution is at least as good as that obtained with comparable phenyl columns.

Sartorius’ Sartobind Phenyl has a binding capacity of 0.4 mg/cm² (15 mg/ml) and can be used for antibody medium scale capture, particularly in the removal of hydrophobic aggregates that can risk unwanted immune system response when patients are treated with therapeutic proteins. Detergent removal and large molecule/bioparticle purification are other possible applications.

The membrane adsorber is easy to handle, suitable for single and batch use and scalable from the 3 ml Sartobind nano version to the Sartobind 150 ml capsule. It will soon also be scalable to the Jumbo 5 l unit.