The water extract of Chinese herbal medicines has complex components and often contains impurities, such as protein, pectin, tannin, starch, et al, which adversely affect the quality, treatment results, dosing, expiration period, and may even be harmful to human health if not removed. The traditional Chinese herbal water extract purification methods, including alcohol precipitation, high speed centrifugation, etc., normally have many drawbacks. Due to the advantages of no solvent consumption, excellent clarity, high recovery rate, short and simple process, low cost and low energy consumption, the flocculation technology has been gaining more and more attention for Chinese medicine purification. This research focused on development of a flocculation technology to purify SBWE, in which the effective component is baicalin and the main impurity is protein. The optimal conditioned flocculation process was determined by using the baicalin retention rate (BRR), protein removal rate (PRR)and the supernatant turbidity (ST) as the evaluation indexes. The action mechanism between the flocculants and the effective component or impurity was analyzed as well.
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