New Affinity Matrix improves protein purification

BAC explains that CaptureSelect is a new purification resin for C-terminal EPEA tagged proteins combining highly selective binding, even under denaturing conditions, with mild elution of the target using the shortest affinity tag system available.The BAC CaptureSelect C-tag affinity matrix combines the selectivity for the shortest affinity tag available, E-P-E-A (glutamic acid-proline-glutamic acid-alanine), with a robust and high quality affinity resin. These properties allow for one-step purification of any C-tagged protein with high purity and yield under both physiological as well as denaturing conditions. In addition, pH sensitive target proteins can be eluted under mild conditions ensuring high activity of purified protein.The CaptureSelect C-tag affinity matrix is specifically designed to simplify the workflow of researchers by reducing both the time and cost of purification, while delivering higher quality output and increased flexibility in the protein purification process. Researchers only need to clone the EPEA sequence at the C-terminal end of the protein of interest. The CaptureSelect C-tag resin provides for a highly specific purification of the tagged protein and is also suitable for use in high throughput processes as well as larger scale packed bed chromatography.