Merck Millipore adds that the Clarisolve® depth filter is a novel clarification device with a gradient density structure specifically designed to the particle size distributions of pre-treated feed streams.Delivering improved volumetric capacity and reduced turbidity over any currently available depth filtration technologies, Clarisolve depth filters process pre-treated feeds in a significantly reduced footprint without the need for the secondary stage of clarification. Merck Millipore adds that the use of Clarisolve depth filters eliminates the need for centrifugation which enables implementation of a fully single-use process train, and also considerably reduces the pre-use flushing requirements. This technology was developed to address the shortcomings of traditional downstream clarification approaches when processing high cell density and high-product titre cell cultures.“Until now, higher titres coming from upstream bioreactors have posed a challenge to conventional approaches to clarification,” described Vin Donovan, vice-president of Biopharm Process Solutions, Merck Millipore. “With Clarisolve depth filters, our customers can now take full advantage of high density pretreated feed streams and improve overall process economics.”Merck Millipore has also developed a cationic pDADMAC (poly diallyldimenthyl ammonium chloride) flocculation reagent targeted for biopharmaceutical applications which can be used as a pre-treatment in combination with the Clarisolve depth filters. pDADMAC treatment, in combination with Clarisolve depth filters, provides an effective solution for harvesting high cell density cultures and can be easily incorporated into current clarification platforms.Clarisolve filters are fully scalable from laboratory to process scale.