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Alfa Laval adds Culturefuge 400 to centrifuge range

27 July 2010

The production-scale centrifuge is the latest addition to Alfa Laval’s range of hermetically designed disc stack centrifuges for harvesting mammalian cell cultures.

The Culturefuge 400 from Alfa Laval handles up to 20 m3/h and consists of a disk-stack high-speed separator with piping for service liquids and process liquids. The centrifuge includes an integrated electrical system with starter, PLC controls and pneumatic unit. A motor with an integrated VFD is standard. A steam-sterilizable pump for transport of the solids phase is optional. The design of the Culturefuge is in accordance with both ASME and PED pressure vessel codes.

The centrifuge incorporates a bottom fed bowl which is completely filled with liquid to ensure that all air is excluded during cell separation. The unique hollow spindle concept allows for gentle acceleration of the feed liquid, which minimises the lysis of shear sensitive cell wall membranes, while the air-free outlet reduces the risk of foaming in the centrate to a minimum.

Culturefuge can be sterilized and cleaned-in-place. The ‘dosing ring’ solids-ejecting system ensures low product losses and drier solids, and flushing nozzles spray the outside of the centrifuge bowl, cyclone and the inside of the frame hood for maximum hygiene.

 

 

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